My day...

8:00 a.m.

Protein extract, loading buffer, boiling samples, molecular weight marker, SDS-PAGE, acrilamide, resolving gel, stacking gel, electrophoresis buffer, electrophoresis...

1:00 p.m.

Lunch with a chronometer

2:00 p.m

Western blot, milipore filter paper, Nylon membrane, transfer buffer, blot apparatus, PBST, Poinceau S, Comassie, scanning of the membranes, protein imobilization, Molico non-fat, washing steps, agitation, first antibody incubation...

8:30 p.m

End of the day....

Devo ser doida, não é?